It actually wouldn'tt affect the MP3 at all. The data would be stored in the metadata of the MP3, just like artist, album, and other song data is stored.
So, long story short, you don't need to make a backup copy of all your MP3s before changing them on the Rolly :)
You *should* back up your MP3s, of course. Most online stores won't let you download your songs again, no matter whether your hard drive crashed or not. So, make sure you back up everything that's important to you! (yeah, I know, it's a rant, but it's TRUE!)
Best,
-Auri